Jun Huang, PhD - 2015 Young Investigator Award
“Single-cell Sequencing to Define the Sequences, Phenotypes and Functionalities of Tumor Antigen Specific CD8+ T Cells from Lymphoma Patients”
Awarded a $75,000 Young Investigator Award
President Obama announced the ‘precision medicine initiative’ in January of 2015, and this proposed research will focus on ‘precision T-cell immunotherapy to cancer.’ CD8+ killer T cells are major mediators of anti-tumor immunity. However, little is known about how CD8+ killer T cells respond to cancer cells, especially their number, frequencies, specificities, sequences, phenotypes and functionalities in cancer patients. Thus, this projects aims to precisely measure these important properties at the single-cell level. Investigators will first use their newly developed high-avidity peptide-MHC dodecamer reagents to sensitively and specifically detect, quantify and isolate tumor antigen-specific CD8+ T cells from lymphoma patients. They will then further apply state-of-the-art single-cell sequencing technologies to simultaneously determine the T cell receptor sequences and functional phenotypes of these CD8+ T cells at the single-cell level. The quantitative knowledge acquired from this study is critical to the development of the next generation of precision medicine for cancer immunotherapy.
"I owe a great deal to the Cancer Research Foundation for giving me a head start. This financial help made the difference between my getting a fast and successful start, and my other wise struggling to obtain the necessary funds to get my research program started."Elaine Fuchs, Ph.D.
Professor, Departments of Medicine and Molecular Genetics and Cell Biology and Human Genetics University of Chicago
April 30, 2010